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Gel shift (EMSA) assay of the PE region using purified His6-tagged RteC protein. The tagged gene was shown to be active in vivo (Table 3, line 7). The DNA substrate used for EMSA was the 260-bp PE fragment that extended from bp +160 to −100 (ATG of rteC). The fragment was end labeled with 32P. The concentrations of the His6-tagged RteC added to the reaction mixtures in μg of protein are shown above each well. “0” indicates no RteC was added.
