TABLE 3.
Effects of RteA/RteB and/or RteC on the GUS activity of the PE-uidA fusion
| BT4001a (pPE-uidA fusion) | + rteC plasmidb | GUS activityc (U/mg of protein)
|
|
|---|---|---|---|
| −Tc | +Tc | ||
| ΩQAB | 1.4 | 1 | |
| ΩQABC | 1 | 250 (+/−42) | |
| ΩQAB | pPc-rteC | <1 | 438 (+/−86) |
| ΩQAB | pPq-rteC | 447 (+/−38) | 517 (+/−70) |
| pPc-rteC | <1 | NAd | |
| pPq-rteC | 668 (+/−76) | NA | |
| pPq-rteC (His6 tagged) | 429 (+/−46) | NA | |
a
BT4001 strain containing the pPE-uidA fusion vector with and without a copy of tetQ-rteA-rteB (ΩQAB) or tetQ-rteA-rteB + rteC (ΩQABC) integrated in the chromosome.
b
BT4001 strain in column 1 with a plasmid carrying rteC controlled by its native promoter (pPc-rteC), rteC controlled by the tetQ promoter (pPq-rteC), or the His6-tagged form of rteC controlled by the Pq promoter.
c
See Materials and Methods for details. Values are the averages of two separate assays of four independent clones, uninduced (−Tc) or induced with 1 μg/ml tetracycline (+Tc).
d
NA, nonapplicable because there is no tetracycline resistance gene (tetQ) in the host strain.