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B. bronchiseptica btrV(S55A), btrV(S55D), btrW(N51A), and btrW(D82AG84A) mutants are defective in type III secretion. Pellet and supernatant fractions of B. bronchiseptica wild-type and various mutant strains defective in type III secretion were separated by SDS-PAGE, transferred to PVDF membranes, and probed with polyclonal antibodies generated against Bsp22 and BopD.
