TABLE 3.
PmtaC1-dependent reporter gene expression in spontaneous M29 mutants
| Strain | Specific β-glucuronidase activitya in:
|
|
|---|---|---|
| Methanol | TMA | |
| J29 | 383 ± 47 | 3.0 ± 0.3 |
| M29 | 570 ± 78 | n.d. |
| M29mut 1 | 315 ± 40 | 388 ± 34 |
| M29mut 2 | 555 ± 52 | 644 ± 59 |
| M29mut 3 | 580 ± 61 | 475 ± 23 |
| M29mut 4 | 561 ± 60 | 637 ± 60 |
| M29mut 5 | 536 ± 49 | 455 ± 41 |
| M29mut 6 | 375 ± 40 | 371 ± 47 |
| M29mut 7 | 485 ± 51 | 520 ± 56 |
| M29mut 8 | 492 ± 50 | 459 ± 47 |
| M29mut 9 | 504 ± 38 | 621 ± 60 |
| M29mut 10 | 391 ± 46 | 482 ± 51 |
a
Specific β-glucuronidase activities are given in mU (nmol × min−1 × mg−1) and are averages of at least three independent experiments. Strains were pregrown for approximately four generations on the respective substrate. n.d., not determined (the strain cannot grow on TMA without a suppressor mutation).