FIG. 4.

RT-PCR analysis for the expression of the M. tuberculosis pknE gene. A 447-bp PCR product corresponding to the internal DNA fragment located between positions 1188 and 1634 bp of the pknE coding sequence was amplified by PCR from the cDNAs of the wild type (WT) but not from the ΔpknE mutant and the negative control reactions. Expression of a 451-bp sigA-specific PCR product, corresponding to the 542- to 992-bp region of the sigA gene, was amplified from the RNA of both the ΔpknE mutant and its parental strain.