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Intracellular growth of the pknH strains in THP-1 cells. Monolayers of differentiated THP-1 cells were infected with the pknH strains for 20 h, and CFU of intracellular bacteria were determined on the indicated days postinfection. The bars represent CFU means and standard deviations obtained from triplicate infections. The mean CFU differences between the ΔpknH mutant and its parental wild-type strain for days 4 and 7 are statistically significant (P < 0.05).
