TABLE 2.

Real-time PCR analysis of gene expression following ethambutol treatment^a

Gene	Ratio of gene/sigA
	Wild type	ΔpknH mutant	Complement
embC	1.96 ± 0.21	0.60 ± 0.15	1.91 ± 0.37
embB	1.33 ± 0.30	0.56 ± 0.02	2.03 ± 0.09
iniB	1.91 ± 0.60	0.16 ± 0.05	2.40 ± 0.24
iniA	1.40 ± 0.42	0.52 ± 0.16	2.09 ± 0.41

^aReal-time PCR analysis was used to quantify the amounts of gene-specific RNAs obtained from untreated and ethambutol-treated cultures (0.2 μg/ml ethambutol for 24 h), and the values obtained were normalized to the levels of sigA RNA. Data presented are mean ± standard deviation (n = 3) induction (n-fold) of gene expression following ethambutol treatment. The values obtained for the ΔpknH mutant strain are significantly different (P < 0.01) from those of the wild type and the complement.