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. 2005 Aug;187(16):5614–5623. doi: 10.1128/JB.187.16.5614-5623.2005

FIG. 3.

FIG. 3.

(A) Primer extension analysis of clpL2, groES, and grpE mRNAs. Total RNA was extracted from O. oeni cells harvested in the exponential phase (lane C) or after a 30-min shift to 42°C (lane HS). Primer extension products are shown alongside DNA-sequencing reaction products (lanes T, G, C, and A). (B) Nucleotide sequences of the clpL2, groESL, grpE, ctsR, hsp18, clpX, and clpP promoter regions. Potential −35 and −10 sequences are underlined and boldface, transcriptional start points are indicated by +1, and CtsR heptad direct-repeat operator sequences are indicated by arrows. Mutation of the groES promoter performed by insertion of a PstI site in the repeated sequence of the CtsR operator site is indicated.