TABLE 1.
Oligonucleotides used in this study
| Name | Sequence (5′ → 3′)a | Created restriction site | Plasmid construction and function |
|---|---|---|---|
| Transcriptional fusion constructions | |||
| olcg11 | GGGGAATTCAATTGAGTGACGGACAAAAGAT | EcoRI | pDLctsR |
| olcg12 | GGGGGATCCCGCTTCGTTTAATCTCGGCG | BamHI | pDLctsR and primer extension on ctsR |
| olcg16 | ATCGGCGGATCCTATCAAATACCTCCTATTAACTAA | BamHI | pDLhsp18 |
| olcg20 | GCCCGAATTCTAAATTAATCGAAGCCTTTTGAC | EcoRI | pDLhsp18 |
| olcg3 | GGGGAATTCCCATTGGGGAGAGGCCGGC | EcoRI | pDLgrpE |
| olcg4 | GGGAAGCTTGCTTGGATCCTTTCACTGCT | HindIII | pDLgrpE and primer extension on grpE |
| olcg14 | CCCGAATTCTTTCGTTGATAATTTTGAAAACATT | EcoRI | pDLgroES and pDLgroESmut |
| olcg15 | CCCGGATCCTATGTAAAACCTCCTTTTAATTTG | BamHI | pDLgroES and pDLgroESmut |
| groES4 | CTACGGCTGCAGAAAAGGTCAAACTTTTTGACAAAG | PstI | pDLgroESmut |
| groES5 | CGGACTCTGCAGAATTGCGTTATAATTTTACGCGG | PstI | pDLgroESmut |
| clpE1 | CCCGAATTCGACAATTTGAGAATCTCTGACCA | EcoRI | pDLclpL2 |
| clpE2 | CCCGGATCCCTGAACCGTTATTTGCTTGTTG | BamHI | pDLclpL2, QRT-PCR and primer extension on clpL2 |
| olcg7 | GGGGAATTCCCCGGAGGCCAGTTTAAGC | EcoRI | pDLclpP |
| olcg8 | GGGGGATCCCCTTGAACCAAGATAATTCGG | BamHI | pDLclpP |
| olcg9 | GGGGAATTCGGATGCTTTTGAAGATGCCG | EcoRI | pDLclpX |
| olcg40 | CCCGGATCCTGATTCTACGTCCATGCTTTTT | BamHI | pDLclpX |
| olcg17 | ATCGGCGAATTCATAAGATAATTTTATCTCTTTTATAAG | EcoRI | pDLmleA |
| olcg18 | ATCGGCGGATCCATTTTCTCCTCAAGAACCACAT | BamHI | pDLmleA |
| RNA analysis | |||
| olcg1 | ATGCATGCCATGGCAGAAGCTAATATTTCAG | RT-PCR, QRT-PCR on ctsR and DNA probe | |
| ctsR1 | AAACGGGTGTTGATTACATAATT | RT-PCR, QRT-PCR, primer extension on ctsR and DNA probe | |
| clpCtsR2 | TTTCATTTCTAATATCATCGTCG | RT-PCR on ctsR-clpC | |
| clpE3 | ATTATAATGACGATCCCTTCGT | QRT-PCR on clpL2 | |
| clpE4 | TGTCCAAACGGATAACCTCC | Primer extension on clpL2 | |
| groES2 | TTCCGCCAACCTTTTCTTCTT | Primer extension on groES | |
| groES3 | TAACAGACTTGGGAGCTTTTG | Primer extension on groES | |
| groES6 | GCCACAACAGAACCCATCACTGGTT | QRT-PCR on groES | |
| groES7 | GGCGATCGAATTGTTCTTAGTAT | QRT-PCR on groES | |
| grpE7 | CGCAGGCAGAAAAGAACAATC | QRT-PCR on grpE | |
| grpE8 | GCTGAAGACGAAGCAGTTGC | QRT-PCR and primer extension on grpE | |
| ldhD1 | GCCGCAGTAAAGAACTTGATG | QRT-PCR on ldhD | |
| ldhD2 | TGCCGACAACACCAACTGTTT | QRT-PCR on ldhD |
a
Specific restriction sites are underlined.