TABLE 1.
Primers used in this study
| Gene and primer | Positionsa | Sequence (5′ to 3′) |
|---|---|---|
| strR | ||
| gs-F | −482 to −463 | GCGGCACGTATGGCCTCCAG |
| gs-R | +218 to +199 | CGACATCCTCGCCGGCACTG |
| fAa-F | −184 to −162 | AATGAAATCGCTGACAGGCGGTG |
| fAa-R | +19 to −4 | AGAGCAATGCTTTCGCACTTCGC |
| fAs-F | −127 to −107 | GTTCCGGTCCTCTCCGCCCTG |
| fAs-R | +77 to +53 | TGTTCCCTGAAATATGCTCCATTAC |
| fBa-F | −394 to −373 | TGAGGCGGGTTCCTGTGCCGCC |
| fBa-R | −200 to −223 | AACCGCAGTTTGATTGCCGAATAC |
| fBs-F | −344 to −324 | TCCTCGCGTGGTCTTGGGCCG |
| fBs-R | −138 to −158 | AGCCAGCGCCTGCGCCCGATC |
| pb-F | −225 to −202 | ATGTATTCGGCAATCAAACTGCGG |
| pf-R | +68 to +44 | AAATATGCTCCATTACACACCCTTC |
| hrdB | ||
| hrdB-F | −121 to −98 | TCGGCCCATTTCGTCACGTATGAG |
| hrdB-R | +193 to +170 | TCGATGAGCGCCATCACAGACTCG |
| adpA | ||
| adpA-F | −223 to −200 | AGCCCCCGCATCCCTCCGCGGCGA |
| adpA-R | +54 to +31 | ACTCGCGAAGCGCACAGGGAAGTG |
| strB1 | ||
| sB1-F | −198 to −176 | AGCCCTGAACTCCTGAAGCACTG |
| sB1-R | +177 to +156 | AAGGCTCGACTCGGCAGTGCTC |
| strD | ||
| sD-F | −428 to −404 | TGTTCCCTGAAATATGCTCCATTAC |
| sD-R | −8 to −28 | TCCTCGCGTGGTCTTGGGCCG |
| strON | ||
| sON-F | −252 to −231 | ACGGTACGACCAACTGTCCTCG |
| sON-R | +158 to +138 | AACAGGGCCTTCTCCACGGCG |
| stsB | ||
| sB-F | −244 to −220 | ATCCATGATTTCCCTCGATTTCGAG |
| sB-R | +148 to +138 | TCGGCTCCCTGTTCGGCCAGC |
| stsC | ||
| sC-F | −250 to −230 | ACTGCCGGGCATCGCTGACTC |
| sC-R | +158 to +137 | TACGAATCCCGGCCCTGATAGG |
| strV | ||
| sV-F | −250 to −230 | AAGCGCGAGGCTAGGTCGGTC |
| sV-R | +155 to +135 | ACCACCGGGATGAGCGCCTGC |
| strU | ||
| sU-F | −450 to −431 | AAGCGGCCAACCCGGCCGTC |
| sU-R | −34 to −54 | AATGCGACCCGTCGCAGCGGG |
a
The nucleotide positions for strR, hrdB, adpA, and strB1 are given taking their transcriptional start points as +1. Those for strD, stsB, stsC, strV, and strU are given taking the first letters of their start codons as +1. The nucleotide positions for strO and strN are given taking the first letter of the start codon of strO as +1.