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Examples of BMH cross-linking of proteins with two Cys replacements in FliGM. The positions of the Cys replacements are indicated at the bottom. DFB225 is a fliG-null strain, which is flagellated and motile when the fliG gene is provided on the plasmid. Strain RP3098 contains a deletion of the flagellar regulatory genes flhDC and does not assemble flagella even when the fliG plasmid is present. Kd, kilodaltons.
