TABLE 1.
Strains and plasmids used in this study
| Strain | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| BL21 (DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) (Cmr) | Novagen |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 (rK− mK+) relA1 supE44 Δ(lac-proAB) [F′ traD36 proAB laqIqZΔM15] | Promega |
| ACP1008 | JM109 containing pACP1005 | This study |
| ACP1009 | BL21(DE3) containing pACP1005 and pGroESL | This study |
| P. multocida strains | ||
| X-73 | Fowl cholera-causing, wild-type strain; serogroup A, serotype 1 | 42 |
| ACP13 | ΔpnhA::tet(M) (Tetr) | This study |
| ACP17 | ΔpnhA::tet(M) containing pACP1007 | This study |
| Plasmids | ||
| pAL99 | P. multocida-E. coli shuttle vector containing promoter region of P. multocida gene tpiA (Knr) | B. Adlera |
| pET30b | Expression vector (Knr) | Novagen |
| pGroESL | Plasmid containing groEL and groES genes and the T7 lac promoter (Cmr) | 16 |
| pVB101 | pBR322 containing tet(M) gene from Tn916 (Apr Tetr) | 11 |
| pACP1000 | pWSK29 containing entire coding region of pnhA and promoter region (Apr) | This study |
| pACP1002 | pACP1000 containing pnhA::tet(M) mutagenesis cassette (Apr Tetr) | This study |
| pACP1005 | pET30b containing promoterless pnhA gene (Knr) | This study |
| pACP1007 | pAL99 containing pnhA gene from pACP1005 (Knr) | This study |
a
Kind gift from Ben Adler, Department of Microbiology, Monash University, Victoria, Australia.