FIG. 3.

Identification of the Rv2358 binding site. Fragments E and F, internal to fragment B (A), were used in EMSA experiments, but only fragment E was retained by Rv2358 protein (B). (C) Promoter/operator sequence organization in M. tuberculosis and M. smegmatis. Palindrome localization between −35 and −10 sites is significantly conserved in M. tuberculosis and M. smegmatis. The consensus promoter is boxed; transcriptional start point, as mapped by experiments with rapid amplification of cDNA ends, is shown by the curved arrow, and palindrome orientation is shown by straight arrows.