FIG. 5.
Growth of the shuS mutant under microaerobic and anaerobic conditions. Bacteria were spread on L plates containing sodium nitrate, 75 μg EDDA per ml, and 15 μM hemin. Control L plates contained sodium nitrate and 20 μM FeSO4. Plates were incubated either aerobically or in sealed chambers made either microaerobic or anaerobic. The diameter of the colonies was measured after 24-h (aerobic and microaerobic) or 48-h (anaerobic) incubation. The strains used were AKS1/pWKS30 (ShuS+), GLS1/pWKS30 (ShuS−), and GLS1/pDD100 (ShuS−/pShuS). An asterisk indicates that no colonies grew on the plate.