Table 1.
Antibodies and reagents used in the study
| Epitope | Origin | Conjugation | Source | Catalog # | Concentration | RRID |
|---|---|---|---|---|---|---|
| Antibodies | ||||||
| eNOS | Mouse | N/A | BD Biosciences | 610296 | 1/2,000 | AB_397690 |
| p-eNOSS1177 | Rabbit | N/A | Abcam | ab215717 | 1/1,000 | AB_2893314 |
| iNOS | Rabbit | N/A | Abcam | ab15323 | 1/500 | AB_301857 |
| MYH11 | Rabbit | N/A | Abcam | ab124679 | 1/8,000 | AB_10975311 |
| ACTA2 | Mouse | N/A | R&D | RB01 | 1/60,000 | |
| MLC | Rabbit | N/A | Cell Signaling | 3672 | 1/3,000 | AB_10692513 |
| p-MLCS19 | Rabbit | N/A | Cell Signaling | 3671 | 1/1,000 | AB_330248 |
| α1-AR | Mouse | N/A | SCBT | sc-100291 | 1/2,000 | AB_1118870 |
| TBXA2R | Rabbit | N/A | Thermo Scientific | PA5-67751 | 1/1,000 | AB_2691697 |
| GAPDH | Mouse | N/A | Cell Signaling | 97166 | 1/10,000 | AB_2756824 |
| Rabbit IgG | Goat | HRP | Cell Signaling | 7074S | 1/10,000 | AB_2099233 |
| Mouse IgG | Horse | HRP | Cell Signaling | 7076S | 1/10,000 | AB_330924 |
| Name | Source | Catalog |
|---|---|---|
| Other reagents | ||
| Stain-free labeling protein | Invitrogen | A44449 |
| ACh chloride | Sigma | A6625 |
| PE hydrochloride | Sigma | P6126 |
| SNP dihydrate | Sigma | 71778 |
| Iron (II) sulfate heptahydrate | Sigma | 12354 |
| Sodium diethyldithiocarbamate trihydrate | Sigma | D3506 |
| DHE | Cayman Chemical | 12013 |
| Calcium chloride | Sigma | C4901 |
| Sodium chloride | Sigma | S9888 |
| Potassium chloride | Sigma | P3911 |
| Magnesium sulfate heptahydrate | Sigma | M2773 |
| Potassium phosphate monobasic | Sigma | P0662 |
| Sodium bicarbonate | Sigma | S6014 |
| d-glucose | Sigma | G5767 |
| Trizma hydrochloride | Sigma | T3253 |
| EDTA disodium salt dihydrate | Millipore | 324503 |
| l-NAME | Sigma | N5751 |
| 1400W | Sigma | W4262 |
| NPA | Sigma | SML2341 |
| EXP-3174 | Cayman | 15957 |
| SOD | Sigma | S5395 |
| Catalase | Sigma | C1345 |
SNP, sodium nitroprusside; NPA, N(ω)-propyl-l-arginine; SOD, superoxide dismutase; DHE, dihydroethidium; MLC, myosin light chain.