TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Agrobacterium | ||
| C58 | Wild-type strain | 14 |
| At11063 | C58 chvG292::aac1 | 7 |
| A136 | C58; Ti plasmid cured | 35 |
| C58ΔpckA | C58 (ΔpckA) | This study |
| E. coli strain DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 φ80dlacZΔM15 | Invitrogen |
| Plasmids | ||
| pSM243cd | virB::lacZ fusion of pTiA6 in pVK102 | 36 |
| pSM358cd | virE::lacZ fusion of pTiA6 in pVK102 | 36 |
| pWT160 | virG::lacZ fusion | 33 |
| pEX18Gm | GmroriT+sacB+; gene replacement vector from pUC18 | 16 |
| pBBR1MCS4 | Broad-host-range vector | 20 |
| pAB2002 | Vector for lacZ fusion | 3 |
| pSY204 | virG constitutive construction | 19 |
| pLP200 | pBBR1MCS-4 with 1.5-kb pckA fragment | This study |
| pLP201 | pWT200 with lacZ-Gmr fragment | This study |
| pEX18Gm-pckA | Construct for deleting pckA | This study |