FIG. 2.

Mass spectrometry analysis of the de novo synthesized cobamide extracted from Halobacterium. Shown is the MALDI-TOF mass spectrometry analysis of the HPLC-purified cobamide extracted from Halobacterium cells producing corrinoids de novo (A) and authentic Cbl (B). The signals with m/z values of 1,330.3 and 1,330.2 (indicated by asterisks) were consistent with the molecular mass of Cbl (without the upper cyano ligand), where z = +1. No significant signals were detected above an m/z value of 1,500 in either case. Me, methyl.