FIG. 4.
Nutritional studies of Halobacterium sp. strain NRC-1 btu mutants. Cbl-dependent growth of Halobacterium sp. strain NRC-1 mutants in CD liquid medium with the indicated concentrations of Cbl at 37°C is reported as the absorbance at 650 nm (OD650) after 6 days of growth. After 6 days, cells reach maximum density, and this value is indicative of the ability to grow in the given medium. The mean cell densities of duplicate experiments are reported ± the standard deviations. The strains are indicated by their genotypes. The strains used were MPK414 (cbiP+ btuCD+ btuF+), JE6738 (ΔcbiP btuCD+ btuF+), JE7084 (ΔcbiP ΔbtuCD btuF+), JE 7108 (cbiP+ ΔbtuCD btuF+), JE7494 (ΔcbiP ΔbtuCD btuF+ ura3::cbiP+), JE7495 (ΔcbiP ΔbtuCD btuF+ ura3::btuCD+), JE7684 (ΔcbiP ΔbtuCD btuF+ ura3::btuC+), JE7685 (ΔcbiP ΔbtuCD btuF+ ura3::btuD+), JE7681 (cbiP+ ΔbtuF btuCD+), JE7682 (ΔcbiP ΔbtuF btuCD+), JE7683 (ΔcbiP ΔbtuCD ΔbtuF), JE7796 (ΔcbiP ΔbtuF ura3::cbiP+ btuCD+), and JE7797 (ΔcbiP ΔbtuF btuCD+ ura3::btuF+).