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TABLE 3.

Plasmids used in this study

Plasmids	Relevant characteristics^a	Source or reference
pCR-Blunt	Km^r, cloning vector	Invitrogen
pUC18	Ap^r, cloning vector	59
pNZ7101	Cm^r Em^r, vector for construction of L. plantarum gene replacement mutants	8
pNZ7105	Km^r, pCR-Blunt derivative containing 5′-flanking region of srtA	This work
pNZ7106	Ap^r, pUC18 derivative containing 3′-flanking region of srtA	This work
pNZ7104	Cm^r Em^r, pNZ7101 derivative containing pNZ7105- and pNZ7106-derived flanking regions of srtA	This work
pNZ7510	Cm^r Em^r, pNZ7101 derivative containing flanking regions of L. plantarum WCFS1 lp_0373	This work
pNZ7511	Cm^r Em^r, pNZ7101 derivative containing flanking regions of L. plantarum WCFS1 lp_1229 (msa)	This work
pNZ7512	Km^r, pCR-Blunt derivative containing L. plantarum WCFS1 lp_0373	This work
pNZ7513	Km^r, pCR-Blunt derivative containing L. plantarum WCFS1 lp_1229 (msa)	This work
pNZ273	Cm^r, vector containing gusA reporter gene	40
pNZ7514	Cm^r, pNZ273 derivative containing constitutive promoter upstream of gusA reporter gene	This work
pNZ7515	Cm^r, pNZ7514 derivative containing L. plantarum WCFS1 lp_0373	This work
pNZ7516	Cm^r, pNZ7514 derivative containing L. plantarum WCFS1 lp_1229 (msa)	This work

Ap^r, ampicillin resistant; Cm^r, chloramphenicol resistant; Em^r, erythromycin resistant; Km^r, kanamycin resistant.