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pH dependency of the tRNA nucleotidyltransferase assays of B. halodurans NTSFI and NTSFII. Left panel, NTSFI assayed with ATP and CTP; right panel, NTSFII assayed with ATP and CTP. Assays were performed as described in Materials and Methods, and results were corrected by subtracting a zero enzyme control value from each experimental value. Results are expressed as picomoles of AMP or CMP incorporated in a 60-μl reaction mixture.
