TABLE 1.
Primers used in this study
| Primer | Gene or protein | Sequence (5′-3′)a |
|---|---|---|
| I-F1 | NTSFI | CCATGGGTTTGAGGCCTATATC |
| I-R1 | NTSFI | CAACGGTGGCAACAGACAAGGACGC |
| II-F1 | NTSFII | GAGTGAAGAACATCAAGAATC |
| II-R1 | NTSFII | GCGAAGCGGCTCCTCAGGCACTTG |
| I-F2 | NTSFI | TATCACATCGGCCAGAAAACC |
| I-R2 | NTSFI | GCTAGCGGGAGCTTCATGCTGC |
| II-F2 | NTSFII | ATGCTAAGAGGGGTACCTGGTG |
| II-R2 | NTSFII | GATGGCGAAAGGAACGAGTGAAGCC |
| I-F3 | NTSFI | GCAGCATGAAGCTCCGCTAGC |
| I-F4 | NTSFI | GCGTCCTTGTCTGTTGCCACC |
| II-F3 | NTSFII | GGCTTCACTCGTTCCTTTCGC |
| II-F4 | NTSFII | CAAGTGCCTGAGGAGCCGC |
| BS16S | rrnB | CAGAGTGACAGGTGGTGCATG |
| ADoT | GGATCCGAATTCTCTAGAT17 | |
| AD20 | GGATCCGAATTCTCTAGA | |
| ADoCT | GGATCCGAATTCTCTAGAC10T6 | |
| I-F5 | NTSFI | AGAGCAGGTGGACATATGGATGAC |
| I-R5 | NTSFI | TTTTTAACAACGGATCCTTCACGACTCCAC |
| II-F5 | NTSFII | TAAAGGAGTCCGCATATGAGTGAA |
| II-R5 | NTSFII | CCGAGGTTTTCTGGATCCGTATCATAAGGG′ |
| 3896F1 | TNT | GTACCCTGTTTTCATATGCCGAACGCC |
| 3896R1 | TNT | CGGAAGGTCGACGGATCCACCGGCTTCTAC |
a
NdeI and BamHI sites are underlined.