FIG. 4.

Cell surface hydrophobicities of Esp-negative FA2-2(pAT28), Esp-positive FA2-2(pEsp219), Esp N-terminal deletion mutant FA2-2(pNdel), and Esp A-repeat deletion mutant FA2-2(pAdel) grown in TSB containing 0.75% glucose. The bacteria were cultured in TSB containing 0.75% glucose, and the respective cell surface hydrophobicities were measured as described in Materials and Methods. The error bars represent means ± standard errors. Deletion of the N-terminal region of Esp resulted in a significant decrease in the cell surface hydrophobicity, whereas no effect was observed on deleting the A-repeat region. Nevertheless, the cell surface hydrophobicity of FA2-2(pNdel) was significantly greater than FA2-2(pAT28).