FIG. 5.

Schematic of the strategy used to generate a plasmid construct expressing a chimeric Esp N terminus and PrtF protein. The N-terminal region of esp with its signal sequence and putative promoter was amplified using primers Esp103 and Esp202R and ligated in-frame to the PrtF-L1 and PrtF-R1 amplified region of prtF. The ligated product was then cloned into the SacI/XbaI-restricted shuttle vector pAT28 to generate a plasmid pPFEN. P, promoter region; S, signal sequence; N, N-terminal domain; R, repeat region; C, C-terminal domain; and F, upstream fibronectin binding domain and RD2 domains.