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Effect of Esp on biofilm formation when expressed in heterologous hosts. E. faecium strain 617 representing heterologous species (A) and L. lactis strain NZ9700 representing a heterologous genus (B) were transformed with pEsp219 to generate Esp-positive strains NZ9700(pEsp219) and 617(pEsp219). The expression of Esp at the cell surface was confirmed by ELISA. Esp did not enhance biofilm formation by strains 617 and NZ9700 under different glucose conditions tested.
