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Urea unfolding curves at different temperatures and in different buffers. Shown are results with imidazole-HEPES, pH 7.5 (A), Tris-glycine, pH 8.8 (B), and CAPS-NH3, pH 10.4 (C). Concentrations are as follows: EDTA, 2 mM; CaCl2, 1 mM. nd, not determined. Urea gradient was 0 to 6.64 M (left to right), and the direction of electrophoresis is from top to bottom.
