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Effect of low temperature on urea unfolding curves. Shown is TUG-GE at 3°C and 12°C in imidazole-HEPES (pH 7.2)-1 mM CaCl2 or boric acid-borax (pH 8.8)-1 mM CaCl2. Row 1 (N⇆U), TUG-GE for AHA showing unfolding; row 2 (U⇆N), TUG-GE for unfolded enzyme showing folding. Urea gradient was 0 to 6.64 M (left to right), and the direction of electrophoresis is shown from top to bottom.
