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Native gel electrophoresis and urea gel electrophoresis of AHA. Native gel electrophoresis (lanes 1 to 3) and 7 M urea gel electrophoresis (lanes 4 to 7) were performed in Tris-glycine buffer, pH 8.8. Unmodified AHA, lanes 1 and 4; AHA treated with 2.5% BME, lanes 2 and 5; AHA modified with IAA, lanes 3 and 6; AHA modified with IA, lane 7.
