TABLE 1.
MALDI-TOF peptide mass mapping of alkylated AHA using endolysC proteolysis
| Protein sequence | Position of cysteine residues | Theoretical average mass ([M + H]+) (Da)
|
Observed average mass ([M + H]+) (Da)
|
||
|---|---|---|---|---|---|
| IAA | IA | IAA | IA | ||
| 1-27 | C20 | 3,326.7 | 3,325.7 | 3,327.4 | 3,326.3 |
| 28-106 | C74 | 8,537.4 | 8,536.4 | 8,538.6 | 8,538.7 |
| 107-169 | C120, C137 | 7,163.7 | 7,161.8 | 7,164.6 | 7,161.8 |
| 301-334 | C328 | 3,740.0 | 3,739.0 | 3,741.5 | 3,740.4 |
| 335-383 | C335 | 5,659.1 | 5,658.1 | 5,659.7 | 5,659.0 |
| 384-406 | C402 | 2,534.8 | 2,533.8 | 2,534.9 | 2,533.8 |
| 415-443 | C416 | 3,076.4 | 3,075.4 | 3,077.3 | 3,075.2 |