TABLE 2.

Kinetic and thermodynamic properties of AHA activity

Kinetic or thermodynamic parameter	Treatment
	3°C			25°C
	Unmodified	BMEa	IA	Unmodified	BME	IA
kcat (s−1)	840	370	267	2321	1062	713
Km (mg ml−1)	2.3	0.78	1.7	2.9	1.3	5.6
ΔG# (kJ mol−1)	52	54	55	54	56	57
ΔH# (kJ mol−1)	26	13b	33	26	13b	33
ΔS# (J mol−1 K−1)	−93	−149	79	−94	−144	−81

^aBME, 1% in assay mixture.

^bArrhenius plot was nonlinear at all temperatures.