FIG. 1.
CAT activities from sodA::cat fusion and expression of cytosolic SODs of V. vulnificus. (A) pSODAC has an insert in which sodA regulatory DNA is transcriptionally fused to cat and cloned downstream from transcription-translation stop Ω DNA (Smr/Spr) (36). cpxA coding for a sensor of the Cpx two-component system perceiving cell envelope stress and trmH coding for tRNA methyltransferase are shown. (B) Activities of MnSOD and FeSOD in extracts of V. vulnificus cells at logarithmic (L) (A600, ∼2.0) and early stationary (ES) (A600, ∼7.0) growth phases. The same amount of protein (50 μg) was loaded in each lane unless stated otherwise. CAT activities from pSODAC in the wild type harvested at the growth phases indicated are illustrated below the gel.