TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| ATCC 29307 | Type strain | 21 |
| AR | ATCO 29307, Rifr | 23 |
| HLM101 | MO6-24/O, Δfur, fur::aph; Kmr | 23 |
| FSA1 | MO6-24/O, Δfur, ΔsodA; Kmr | This study |
| KPR101 | AR, ΔrpoS | 37 |
| SA1 | AR, sodA::aph; Kmr | This study |
| SB1 | AR, sodB::aph; Kmr | This study |
| SC1 | AR, sodC::aph; Kmr | This study |
| SR1 | AR, soxR::aph; Kmr | This study |
| JR203 | ATCC 29307, cadA::npt; Kmr | 40 |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 12 |
| S17-1 | C600::RP4 2-(Tc::Mu)(Km::Tn7) thi pro hsdR hsdM+recA | 46 |
| S17-1λpir | λpir lysogen of S17-1 | 46 |
| BL21(DE3) | E. coli B F−dcm ompT hsdS(rB− mB−) gal λ(DE3) | Stratagene |
| Plasmids | ||
| pRK415 | ori IncP Mob RP4 lacZa; Tcr | 16 |
| pSODAC | pRK415 + sodA::cat fusion; Tcr | This study |
| pDM4 | ori R6K Mob RP4; Cmr | 32 |
| pDMSAKm | pDM4 + 3.0-kb fragment containing sodA::aph; Kmr, Cmr | This study |
| pDMSAid | pDM4 + ΔsodA; 0.6-kb XbaI-XhoI fragment of pSAid; internally deleted (BglII-StuI) from sodA; Cmr | This study |
| pDMSB | pDM4 + 3.1-kb fragment containing sodB::aph; Kmr, Cmr | This study |
| pDMSC | pDM4 + 3.1-kb fragment containing sodC::aph; Kmr, Cmr | This study |
| pDMSXR | pDM4 + 2.8-kb fragment containing soxR::aph; Kmr, Cmr | This study |
| pRKSA | pRK415 + 0.8-kb fragment containing sodA; Tcr | This study |
| pRKSB | pRK415 + 0.8-kb fragment containing sodB; Tcr | This study |
| pRKSC | pRK415 + 1.1-kb fragment containing sodC; Tcr | This study |