TABLE 2.

Primers used in this study

Primer name	Nucleotide sequence
phoA (for sequencing)	5′-GGTGCAGTAATATCGC-3′
yplA198 (for sequencing)	5′-CCGCCTAATGAATGGCCG-3′
SacII-Pcat	5′-TCCCCGCGGGGAAAGTATCTTCCTGGC-3′
BamHI-yplA-end	5′-GGATCCTCATTCAGGCATACTGCCGC-3′
phoA-KpnI	5′-GGGGTACCCCATCTGCCATTAAGTCTG-3′
BamHI-phoA	5′-CGGGATCCCGCTGACTCTTATACAC-3′
yplA-codon 1	5′-CGGGATCCCTCATGCCAACTCCTTGTCG-3′
yplA-codon 9	5′-CGGGATCCCTCCGGGCTAAACTAAC-3′
yplA-codon 15	5′-CGGGATCCCTGCCTGCTAATGGAACAGG-3′
yplA-codon 20	5′-CGGGATCCCTCACCTGGTTCATCAAGCC-3′
yplA-codon 30	5′-CGGGATCCCTGTTCTCCTTCTTGACCTG-3′
yplA-codon 40	5′-CGGGATCCCTAAGCTGGTTCGAAGACCC-3′
yplA-codon 60	5′-CGGGATCCCTCATGCTGTGTAATAAACATT-3′
yplA-codon 75	5′-CGGGATCCCTGGCAGCTAAAACACGTTC-3′
yplA-codon 105	5′-CGGGATCCCTAGCAGGAGCGTAAACATC-3′
BamHI-yplA11	5′-AGGGATCCCGTTGGATTAGCAGGC-3′
BamHI-yplA21	5′-AGGGATCCCCAACCAATAGAGCAG-3′
BamHI-yplA31	5′-AGGGATCCCTCTACTCCTGTTGGG-3′
BamHI-yplA41	5′-AGGGATCCCCAAAAAGAATCCGCC-3′
YplAΔ2-30-2	5′-CCCAACAGGAGTAGACATGCCAACTCCTTGTCG-3′
YplAΔ2-30-3	5′-GGCATGTCTACTCCTGTTGGG-3′
YplAΔ2-10-2	5′-GCCTGCTAATGGAACCATGCCAACTCCTTGTCG-3′
YplAΔ2-10-3	5′-GGCATGGTTCCATTAGCAGGC-3′
YplAΔ31-40-2	5′-GGATTCTTTTTGAAGGTTCTCCTTCTTGACCTG-3′
YplAΔ31-40-3	5′-GAGAACCTTCAAAAAGAATCC-3′
UTR2	5′-ACTAACAGATATACTCATTTTAGCTTCCTTAGC-3′
UTR3	5′-GCTAAGGAAGCTAAAATGAGTATATCTGTTAGT-3′
Frameshift2	5′-AGGCCGGGCTAAAACTAACAGATATACCATTTTAGCTTC-3′
Frameshift3	5′-GAAGCTAAAATGGTATATCTGTTAGTTTTAGCCCGGCCT-3′
ΔyplAB1	5′-GGATCCCTGATCCAATTCGGTCAG-3′
ΔyplAB2	5′-CGGGCTTCAGGATCAGCAAGATCTCATGCCAACTCCTTGTCG-3′
ΔyplAB3	5′-AGATCTTGCTGATCCTGAAGCCCG-3′
ΔyplAB4	5′-GGATCCCGTTCTGGTTGCAACTGA-3′