FIG. 3.

Steady-state protein levels of Mga produced from both the native and various constitutive promoters. Western analysis (top) was performed on whole-cell lysates using an anti-His antibody for the detection of Mga-His protein in samples either not producing Mga (lane 1) or producing M4 Mga from the constitutive promoters Pspac (lane 2) and PrpsL (lane 4) or the native promoter Pmga4 (lane 3). Amido black stain (bottom) of total protein on membranes was used as a loading control. All blots shown are representative of data from three independent experiments.