TABLE 2.
Plasmid | Relevant characteristic(s) | Reference or source |
---|---|---|
pBluescriptII KS(−) | Blue/white cloning vector | Stratagene |
pET21a | Six-His vector | Novagen |
pMal-c2 | malE MBP fusion vector | New England Biolabs |
pMga4-4 | WTmga4ain pLZ12-Spec | 2 |
pMga50-His | WT mga50 gene with C-terminal six-His tag | This study |
pPmga-blue | Pmga in pBluescript II KS(−) | This study |
pPmga-gusA | gusA under Pmga6 | This study |
pPmrp-gusA | gusA under Pmrp | This study |
pVIT164 | GAS integration vector | 6 |
pJRS312 | Spectinomycin cassette | 26 |
pJRS515 | WT mga6 in pBluescript II KS(−) | 18 |
pJRS547 | Deletion of 2.1 kb of mga and Pmga (mga-16) | This study |
pJRS9160 | Counterselectable gene replacement vector | 20 |
pKSM140 | Promoterless gusA in pCIV2 suicide vector | This study |
pKSM155 | N-terminal MBP fusion to M4 Mga | This study |
pKSM156 | N-terminal MBP fusion to M50 Mga | This study |
pKSM162 | WT mga6 under constitutive Pspac | 20 |
pKSM164 | WT mga6-his under native Pmga6 | 1 |
pKSM174 | mga-16 deletion allele in pJRS9160 | This study |
pKSM315 | WT mga50 under constitutive Pspac | This study |
pKSM318 | WT mga6-his under constitutive Pspac | This study |
pKSM320 | WT mga4-his under constitutive Pspac | This study |
pKSM321 | WT mga50-his under constitutive Pspac | This study |
pKSM317-H | mga50-his (S26N) under constitutive Pspac | This study |
pP361A-H | mga50-his (P361A) under constitutive Pspac | This study |
pR461M-H | mga50-his (R461M) under constitutive Pspac | This study |
pKSM322 | WT mga4-his under native Pmga4 | This study |
pSKM323 | mga50-his (S26N) under native Pmga4 | This study |
pKSM324 | WT mga4-his under constitutive PrpsL | This study |
pKSM325 | WT mga50-his under constitutive PrpsL | This study |
pKSM326 | mga50-his (S26N) under constitutive PrpsL | This study |
pKSM327 | mga50-his (P361A) under constitutive PrpsL | This study |
pKSM328 | mga50-his (R461M) under constitutive PrpsL | This study |
pKSM329 | WT mga50-his under native Pmga4 | This study |
pKSM330 | mga50-his (R461M) under native Pmga4 | This study |
pKSM331 | mga50-his (S26N/R461M) under native Pmga4 | This study |
pKSM332 | mga50-his (S26N/R461M) under constitutive PrpsL | This study |
pKSM333 | WT mga6-his under constitutive PrpsL | This study |
pKSM336 | mga50-his (P361A) under native Pmga4 | This study |
pKSM337 | mga6-his (M461R) under constitutive Pmga6 | This study |
pKSM338 | mga6-his (M461R) under constitutive PrpsL | This study |
WT, wild type.