TABLE 2.
Spots only present in gels from extracts of cultures grown in the presence of toluene
| Spot(s) | Protein | Gene | Accession no.a | Protein mol wt (103) |
|---|---|---|---|---|
| 638, 636, 1184, 1180 | Toluene 2,3-dioxygenase (α subunit) | todC1 | PPUY18245 | 51.3 |
| 1339 | 2-Hydroxy-6-oxo-2,4-heptadienoate hydrolase | todF | PPUY18245 | 30.8 |
| 603 | Succinate-semialdehyde dehydrogenase | gabD | PP0213 | 51.8 |
| 1227 | Succinyl-coenzyme A synthetase (β subunit) | sucC | PP4186 | 41.5 |
| 563 | Oxoglutarate dehydrogenase, lipoamide dehydrogenase component | lpdG | PP4187 | 50.1 |
| 1232 | Fructose-1,6-bisphosphate aldolase | fda | PP4960 | 38.7 |
| 1140 | Phosphoenolpyruvate carboxykinase (ATP) | pflu3720b | Pflu3720 | 58.3 |
| 1401 | Inorganic pyrophosphatase | ppa | PP0538 | 19.1 |
| 1808, 1222, 1203, 1209 | Translation elongation factor Tu | tuf-1 | PP0440 | 43.8 |
| 1238 | ABC transporter, periplasmic binding protein | PP1726 | 38.6 | |
| 1480 | Cold shock protein CspA | cspA2 | PP2463 | 7.7 |
a
PP numbers are based on the locus of the genome of Pseudomonas putida KT2440. PPUY numbers are the accession numbers of the TodC1 and TodF nucleotide sequences of P. putida DOT-T1E deposited in GenBank.
b
Based on the protein identified in Pseudomonas fluorescens PfO-1.