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. 2005 Sep;187(17):6187–6196. doi: 10.1128/JB.187.17.6187-6196.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 2. — Requirement of mreBCD for partial complementation of morphological and IcsA localization phenotypes of mreB cells. Bright-field (panels A to D and E, bottom), DIC (panel F, bottom), and fluorescence (panels E and F, top) microscopy of live E. coli mreB cells, with or without complementation with mreB or mreBCD in trans. (A) Rod-shaped wild-type strain. (B) Spherically shaped MC1000 ΔmreB::FRT-cat-FRT. (C) MC1000 ΔmreB::FRT-cat-FRT expressing mreBCD in trans. (D) MC1000 ΔmreB::FRT-cat-FRT expressing mreB in trans. (E) MC1000 ΔmreB::FRT-cat-FRT expressing mreBCD and IcsA_507-620-GFP. (F) MC1000 incubated with the MreB inhibitor A22 for 2 h and expressing IcsA_507-620-GFP. Size bar, 5 μm.