Table 1.

Primers and Probes Used for Quantitative Real-Time Polymerase Chain Reaction

TTF-1

Forward primer (TTF1-F1): 5′-AAG TTC TCA CAG ATC AGC AGT CAA AG-3′

Reverse primer (TTF1-R1): 5′-TTC TTC GAC AGC CTT GAT TAG TTT C-3′

Probe (TTF1-P1): 5′-/56-FAM/GGG TTT CAG ACT TAC TCC AAG CAC CAC CAC GAT/3BHQ_1/-3′

TTF-2

Forward primer (TTF2-F1): 5′-CCA GCA CCG TCC ACA TAC TGT-3′

Reverse primer (TTF2-R1): 5′-CCA GGG CCG ACT TCA GTA AAG-3′

Probe (TTF2-P1): 5′-/56-FAM/CCA GTT GCT GAG ACT CCG CCA GTG TT/3BHQ_1/-3′

Pax8A

Forward primer (Pax8A-F1): 5′-CAG CCT GGC AGC GAC AA-3′

Reverse primer (Pax8A-R1): 5′-GCT GCT CTG TGA GTC AAT GCT TAG-3′

Probe (Pax8A-P1): 5′-/56-FAM/CAG CTA TCC TGA TCA CTG TCA TCC ATT TTC CT/3BHQ_1/-3′

TSHR

Forward primer (TSHR-F1): 5′-GAC CCT GAT GCC CTC AAA GA-3′

Reverse primer (TSHR-R1): 5′-TCA GGG AAC ATT TTA AGT CCA GTG T-3′

Probe (TSHR-P1): 5′-/56-FAM/CTC CCC CTC CTA AAG TTC CTT GGC ATT T/3BHQ_1/-3′

TTF, thyroid transcription factor; TSHR, thyrotropin receptor.