Table 2.
Antibodies.
| Species | Source | Cat. no., RRID | Method, dilution | Antigen (kDa) | |
|---|---|---|---|---|---|
| Primary antibody | |||||
| cFos | Rabbit | Santa Cruz Biotechnology | Cat# sc-52, RRID: AB_2106783 | IHC, 1:500 | – |
| EGFP | Mouse | Sigma-Aldrich | Cat# G6795, RRID: AB_563117 | IHC, 1:1000 | – |
| HA.11 | Mouse | BioLegend | Cat# 901515, RRID: AB_2565334 | IHC, 1:750 | – |
| β-actin | Mouse | Sigma-Aldrich | Cat# A1978, RRID: AB_476692 | WB, 1:1000 | 40 |
| GAD 65/67 | Mouse | Enzo Life Sciences | Cat# ADI-MSA-225-E, RRID: AB_2039129SA-225E | WB, 1:1000 | 65/67 |
| GluA1 | Rabbit | Alomone Labs | Cat# AGC-004, RRID: AB_2039878 | WB, 1:1000 | 100 |
| GluA1 phS831 | Rabbit | Rockland | Cat# 612-401-C82, RRID: AB_11181727 | WB, 1:1000 | 100 |
| GluN2B | Rabbit | Millipore | Cat# 06-600, RRID: AB_310193_310193 | WB, 1:1000 | 180 |
| GluN2B phY1472 | Rabbit | Sigma-Aldrich | Cat# M2442, RRID: AB_262150 | WB, 1:1000 | 180 |
| Synapsin-1 | Mouse | Synaptic Systems | Cat# 106001a, RRID: AB_2617071 | WB, 1:1500 | 78 |
| Synaptotagmin-2 | Rabbit | Synaptic Systems | Cat# 105123, RRID: AB_2199465 | WB, 1:1000 | 70 |
| Synaptophysin 1 (p38) | Mouse | Synaptic Systems | Cat# 101011C2, RRID: AB_10890165 | WB, 1:1000 | 38 |
| PSD 95 | Rabbit | Synaptic Systems | Cat# 124002, RRID: AB_887760 | WB, 1:1000 | 95 |
| Secondary antibodies | |||||
| Anti-mouse biotinylated | Goat | Agilent | Cat# E0433, RRID: AB_2687905 | IHC, 1:750 | – |
| Anti-mouse Alexa 568 | Donkey | Abcam | Cat# ab175472, RRID: AB_2636996 | IHC, 1:1000 | – |
| Anti-rabbit Alexa 568 | Donkey | Abcam | Cat# ab175470, RRID: AB_2783823 | IHC, 1:1000 | – |
| Anti-rabbit Alexa 488 | Donkey | Abcam | Cat# ab150073, RRID:AB_2636877 | IHC, 1:1000 | – |
| Anti-mouse-AP | Rabbit | Dako A/S | Cat# D0314 | WB, 1:5000 | – |
| Anti-rabbit-AP | Goat | Agilent | Cat# D0487, RRID: AB_2617144 | WB, 1:2000 | – |
| ABC reagent | – | Vector Laboratories Inc. | Cat# PK-7100, RRID: AB_2336827 | As recommended | – |