. 2005 Aug;49(8):3101–3108. doi: 10.1128/AAC.49.8.3101-3108.2005

TABLE 1.

Oligonucleotides used in this study

Primer	Sequence (5′ to 3′)	Annealing temp (°C)
FCa^a	TGGGCNGGNTGYYTNGT	53
FCb^a	TACCANGTYTGRTTCAT	53
YPa	TGGAGAAAGGGAAGTTTTGCTTCTTACGAC	67
YPb	GTCGTAAGAAGCAAAACTTCCCTTTCTCCA	67
YNa	GGTGTGGTTTTGGGTATGAACCAGACGTGG	67
YNb	GGCAATCAAGTTCATGAAGTTCTCCATGAC	67
YP1	TTCAGAAGAGGATTCAAGGGCTACAATCCG	67
YP2	CGGATTGTAGCCCTTGAATCCTCTTCTGAA	67
YN1	TGCACGGTTCTTACTATGAACCAAACCTGG	67
YN2	ACCGATAATACTCAAGAAGTTCGACATGGT	67
FC1	AGCGAAGGGTTGAGTTGC	64
FC2	ATGTCGAGTTCCGTTCTC	64
FC3	CTGGTAGAATACAACACCG	60
FC4	CGGAACGTACGAGAGAGAC	60
FciS^b	TCGAGAACTAGTGGCGAAACAAAGAAGAGA	60
FciN^c	CGAGCTATGCATACCAAACCACCAACAGAC	60
Fcy2S^b	TCGAGAACTAGTGATGGAAAGGAGAGGCTG	62
Fcy2N^c	CGAGCTATGCATAGATTTGCTACTCTGATG	62
18S1	TTTCGATGGTAGGATAGAGGC	62
18S2	CATGCTAACAGATTCAAGCGG	62

For degenerate primers FCa and FCb, D is A, G, or T; H is A, C, or T; R is A or G; W is A or T; and Y is C or T.

The SpeI restriction sites located in primers FciS and Fcy2S are indicated in boldface type.

The NsiI restriction sites located in primers FciN and Fcy2N are indicated in boldface type.