TABLE 4.
Pyocin/phage operon and related genes induced by 0.3× and 1× MIC of ciprofloxacina
| ORF | Gene nameb | Change (n-fold) with 0.3× MIC | Change (n-fold) with 1× MIC | Description |
|---|---|---|---|---|
| PA0610 | prtN | 2.0 | 6.8 | Transcriptional activator |
| PA0611 | prtR | 1.3 | 3.0 | Transcriptional repressor |
| PA0612 | —c | 8.5 | Homolog of Zn2+ finger protein | |
| PA0613 | 4.0 | 19.3 | Conserved hypothetical | |
| PA0614 | Hol | 3.3 | 15.3 | Holin |
| PA0615 | 1.7 | 7.2 | Conserved hypothetical | |
| PA0616 | VR2 | 2.9 | 13.0 | Homologous to baseplate assembly protein V |
| PA0617 | WR2 | 3.0 | 13.9 | Homologous to baseplate assembly protein W |
| PA0618 | JR2 | 2.1 | 10.0 | Homologous to baseplate assembly protein J |
| PA0619 | IR2 | 3.2 | 14.7 | Homologous to tail protein I |
| PA0620 | HR2 | 2.7 | 12.1 | Homologous to tail fiber protein H |
| PA0621 | 4.3 | 16.7 | Homologous to tail fiber assembly protein | |
| PA0622 | FIR2 | 2.4 | 10.3 | Homologous to contractile sheath protein FI |
| PA0623 | FIIR2 | 2.9 | 12.8 | Homologous to tail tube protein FII |
| PA0624 | 2.7 | 12.8 | Conserved hypothetical | |
| PA0625 | 2.5 | 14.1 | Homologous to tail length determination protein | |
| PA0626 | UR2 | 1.8 | 9.8 | Homologous to tail formation protein U |
| PA0627 | XR2 | 2.3 | 10.8 | Homologous to tail protein X |
| PA0628 | DR2 | 1.8 | 10.0 | Homologous to tail formation protein D |
| PA0629 | Lys | 2.2 | 8.7 | Lytic protein; homology to predicted chitinase |
| PA0630 | 1.6 | 10.6 | Hypothetical protein | |
| PA0631 | 2.4 | 16.2 | Unique hypothetical protein | |
| PA0632 | 3.4 | 21.2 | Unique hypothetical protein | |
| PA0633 | VF2 | 4.3 | 16.4 | Homologous to major tail protein V |
| PA0634 | 3.2 | 15.8 | Unique hypothetical protein | |
| PA0635 | 2.0 | 15.9 | Conserved hypothetical protein | |
| PA0636 | HF2 | 2.1 | 10.0 | Homologous to tail length determination protein H |
| PA0637 | MF2 | 2.7 | 11.0 | Homologous to tail fiber protein M |
| PA0638 | LF2 | — | 8.5 | Homologous to tail fiber protein L |
| PA0639 | KF2 | 3.0 | 15.9 | Homologous to tail assembly protein K |
| PA0640 | IF2 | 3.1 | 16.4 | Homologous to tail assembly protein I |
| PA0641 | JF2 | 2.4 | 9.8 | Homologous to tail fiber protein J |
| PA0642 | 2.7 | 12.9 | Hypothetical protein | |
| PA0643 | 1.9 | 8.9 | Homologous to tail fiber domain protein | |
| PA0644 | 4.0 | 18.0 | Hypothetical protein | |
| PA0645 | 1.8 | 15.8 | Hypothetical protein | |
| PA0646 | 3.1 | 13.4 | Homologous to putative tail fiber protein | |
| PA0647 | 3.8 | 23.0 | Conserved hypothetical protein | |
| PA0648 | — | 8.2 | Conserved hypothetical protein | |
| PA0985 | pys5 | 4.5 | 18.1 | Pyocin S5 |
| PA1150 | pys2 | — | 5.4 | Pyocin S2 |
| PA3617 | recA | 2.8 | 4.6 | Recombinase for DNA recombination and repair |
| PA3866 | pys4 | 13.9 | 51.3 | Pyocin S4 |
Only genes identified as being affected by subinhibitory (0.3 μg/ml) or inhibitory (1 μg/ml) ciprofloxacin relative to untreated strain H103 and that were of interest in this study are included. A list of all genes identified as being affected by this concentration of ciprofloxacin and exhibiting a statistically significant change (P ≤ 0.05) is available in the supplemental material. Genes are identified by ORF designation, gene name or alternative gene name, and homology description based on the Pseudomonas genome project (www.pseudomonas.com).
Genes were named as per Table 1 of Nakayama et al. (21) and reflect the homology observed by these authors to phages P2 and λ.
—, no significant change in expression.