TABLE 1.

Reduction of SM101 inoculum in mouse blood by acpP-targeting PNA^a

Treatment	No. of mice bled	No. of CFU/ml
SM101 + no PNA	6	0, 0, 6 × 103, 1.6 × 103, 4.6 × 103, >105
SM101 + 5-nmol PNA	4	0, 0, 0, 0
SM101 + 50-nmol PNA	4	0, 0, 0, 0

^aMice were infected with an LD₇₀ of E. coli SM101. Treatment with acpP-targeting PNA occurred in vivo 30 min prior to infection. Two-hundred-microliter blood samples were collected at 24 h after SM101 challenge, and bacteria were enumerated. Samples were diluted and plated on LB plates and incubated overnight at 37°C, and colonies were assessed by visual inspection.