TABLE 2.

Reduction of proinflammatory cytokines in serum by acpP-targeting PNA^a

Treatment	TNF-α	IL-1β	IL-6	IL-12
SM101 + no PNA	0.86 ± 0.08	0.43 ± 0.33	12.16 ± 3.08	4.58 ± 0.01
SM101 + 5-nmol PNA	0.58 ± 0.18	0.50 ± 0.12	0.05 ± 0.01b	0.62 ± 0.07b
SM101 + 50-nmol PNA	0.54 ± 0.03b	0.31 ± 0.07	0.04 ± 0.03b	0.44 ± 0.06b

^aMice were infected and treated as described in the footnote to Table 1. Serum collected 24 h post SM101 challenge was examined for proinflammatory mediators TNF-α, IL-1β, IL-6, and IL-12 by standard ELISA. Treated mice demonstrated marked reductions in TNF-α, IL-6, and IL-12. Data are presented as μg/ml (± standard deviation) of mediator in serum. ELISA values were assessed in duplicate. Similar results were obtained in repeat experiments.

^bP < 0.05 relative to controls as determined by Student's t test.