FIG. 1.

Activity of WLBU2 in human plasma. To determine activity in human plasma, bacterial isolates of P. aeruginosa PAO1 (∼10⁶ cells/ml) were incubated with twofold serially diluted peptides at 37°C for 30 min either in heat-inactivated human plasma or in serum. Bacterial survival at corresponding peptide concentrations was evaluated by broth dilution assays as previously described. WLBU2 was able to sterilize the bacterial suspension at 12.5 μM, while LL37 demonstrated no significant activity even at 100 μM (not shown). Results of activity in serum and plasma were identical. The graphs were derived from average values of four independently generated and almost identical triplicate sets of data.