TABLE 3.

Comparison of susceptibilities of Campylobacter parent and mutant strains in the putative efflux pump inactivation study

Straina	Method of transformation	MIC (μg/ml)b
		CHL	CIP	ERY	TET
124	NA	16	128	32	512
124-Cj0035c	Natural	64	128	32	512
124-CmeB-Kan	Natural	2 (8)	4 (32)	0.5 (64)	8 (64)
124-Cj1033	Natural	32	128	32	512
124-Cj1257c	Natural	32	128	32	512
241	NA	8	16	512	256
241-Cj0035c	Natural	32	16	512	256
241-Cj0310c	Natural	32	16	512	256
241-CmeB	Electroporation	32 (NA)	4 (4)	2 (256)	16 (16)
241-Cj0619	Natural	32	16	512	256
241-Cj1033	Natural	16	16	256	256
241-Cj1174	Electroporation	32	16	512	256
81-176	NA	4	0.125	2	32
Cj0035c	Electroporation	32	0.125	2	32
Cj0310c	Electroporation	32	0.125	2	32
CmeB	Electroporation	16 (NA)	0.03 (4)	0.125 (16)	8 (4)
CmeB-Kan	Natural	2 (2)	0.03 (4)	0.125 (16)	8 (4)
Cj0560	Electroporation	32	0.125	2	32
Cj0619	Electroporation	32	0.125	2	32
Cj1033	Electroporation	16	0.125	2	32
Cj1033-Kan	Electroporation	4	0.125	2	32
Cj1174	Electroporation	32	0.125	2	32
Cj1241	Electroporation	16	0.125	2	32
Cj1257c	Electroporation	16	0.125	2	32
Cj1687	Electroporation	16	0.125	2	32
81-176ery	NA	4	4	16	32
Ery-Cj0035c	Electroporation	16	4	16	32
Ery-Cj0310c	Electroporation	16	4	16	32
Ery-CmeB	Electroporation	16 (NA)	1 (4)	0.125 (128)	8 (4)
Ery-Cj0560	Natural	16	4	16	32
Ery-Cj0619	Electroporation	16	4	16	32
Ery-Cj1033	Electroporation	8	4	16	32
Ery-Cj1174	Electroporation	16	4	16	32
Ery-Cj1241	Electroporation	16	4	16	32
Ery-Cj1257c	Natural	16	4	16	32
Ery-Cj1687	Electroporation	16	4	16	32

^aMutations that resulted in >2-fold MIC changes are in boldface.

^bThe numbers in parentheses indicate the differences (n-fold) in MICs between parents and their mutant derivatives. The breakpoints used were 32, 4, 8, and 16 μg/ml for CHL, CIP, ERY, and TET, respectively.