Figure 9.

Jnm1–Arp1p 2-hybrid interaction and overexpression-rescue of the Arp1p-specific arp1-100/115 projection by Jnm1p and Nip100p. (A) The results of two-hybrid interaction between Jnm1p and mutant Arp1p. Mutation of the Arp1p residues shown in blue decreased the interaction with Jnm1p, those in yellow had no effect. The blue circle indicates the position of the Arp1p-specific projection, encompassing arp1-38, 100, and 115. A schematic representation of the filament consisting of five boxes, each with a magenta inner face and teal outer face, serves as an orienting guide to the relative rotation of each filament. (B) Plasmids overexpressing (2μ His⁺) either JNM1 or NIP100 were introduced into all Ts^– and lethal ARP1 mutant strains, ARP1 wild-type, or arp1Δ. Their ability to rescue the ARP1 mutation was accessed by growth under conditions (5-FOA-HIS) that counterselect the wild-type ARP1 covering plasmid. Though all Ts^– and lethal alleles were examined, only the ARP1 alleles demonstrating rescue are shown.