Table 1.

ARP1 allele position and residues, protein levels, and overexpression rescue

ARP1 allele	Residue	Pheno	PP	PR	CEN	2μ
33	K222A, E223A, K224A	L	-	-	-	-
35	R185A, D187A	T	+	+	(+)	+
36	D321A, R322A	L	-	(-)	-	-
37	E31A, E32A	W	+	+
38	E374A, D375A	T	(-)	(-)	(-)	+
39	E84A, D85A	T	(-)	(-)	+	+
40	D58A	W	+	+
41	D87A, E90A	W	+	+
42	E108A	W	+	+
44	D48A, K49A, E53A	W	(+)	(-)
45	K233A, K234A, E235A, E236A, E237A, K238A	T	(-)	(+)	+	+
46	K46A, D48A, K49A	W	(+)	(-)
85	R266A, E269A	L	+	-	-	-
86	R124A, E125A	W	+	+
94	D2A, D6A	T	-	-	+	+
95	K35A, E38A	W
96	K6A7, R69A	W
97	K73A, R75A	W
98	R75A, K79A	W
99	K122A, R124A, E125A	W
100	E133A, D136A	L	-	-	-	(+)
101	D162A, E165A	W
102	D192A, E195A	W
103	R202A, K203A	W
104	E214A, R215A, E216A	L	-	-	-	(+)
105	K251A, D254A	L/DC	-	-	-	-
106	R256A, E259A	W
107	D263A, R264A	W
108	D282A, D286A	W
109	K294A, D296A	W
110	D298A, R300A, K301A	W
111	D326A, E328A	T	-	-	+	+
112	K336A, K338A	W	+	+
113	E344A, R345A, K346A	T	(-)	(-)	-	-
114	R364A, K368A	W
115	K369A, D371A	L	-	-	-	+
134	E31A, E32A, R33A, K35A	W
135	E214A, R215A, E216A, R219A	L			-	-
136	K230A, K233A, K234A	W
137	D296A, D298A	W
138	K317A, D321A	W
139	K332A, K336A	W
140	E374A, D375A, R378A	T			(+)	(+)
176	Y151A	W
181	Y151S	W

Pheno, phenotype; PP, protein at permissive temperature; PR, protein at restrictive temperature; CEN, rescue by one or two copies of allele; 2μ, rescue by high-copy; L/DC, lethal and dominant cold-sensitive; W, pseudo-wild type; T, temperature sensitive (Ts^-); gray highlights exceptions to general trends. Protein levels (PR, PP) or growth (CEN, 2μ) are indicated, in decreasing level, by +, (+), (-), and -.