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GAP activity of Gcs1p for Arl1p in vitro. GAP activity of the different concentrations of purified recombinant Gcs1p or Glo3p (as indicated at bottom) was assayed using Arl1p (square), Arf1p (circle), or Arl3p (triangle) with [γ-32P]GTP bound as substrate as described in Materials and Methods.
