Figure 6.
More Arl1p is present in trans-Golgi of gcs1 mutant than wild-type yeast. (A) Wild-type, gcs1-, or glo3-deleted yeast were stained with anti-GFP and affinity-purified anti-Arl1p antibodies to monitor the Golgi association of Arl1p. GFP-Sft2p was the late-Golgi marker. (B) Quantification of Golgi association ratio of Arl1p. Signals in A were quantified by Axio Vision Rel. 4.2 software. Punctate signals were summed and divided with whole cell signals (n = 50 and p < 0.05).