The tER and Golgi association of dGRASP is mediated by the same mechanism. S2 cells expressing GFP alone (A), full-length dGRASP-GFP (B), G2A-dGRASP-GFP (C), N-ter-GFP (D), and C-ter-GFP (E) were double-labeled with dSec23 (red, tER) and d120 kDa (blue, Golgi). Note that G2A-dGRASP-GFP and the C-ter-GFP exhibit a cytosolic distribution, whereas the N-ter-GFP is mostly associated with tER-Golgi units similar to the full-length dGRASP. Projections are presented. Bar, 5 μm.