Table 1.
Stereological analysis of dGRASP localization in Drosophila cells and tissues
| Relative distribution
|
Linear density (gold/μm of membrane)
|
||||||
|---|---|---|---|---|---|---|---|
| % of membrane-associated gold particles | Golgi | tER | ER | Golgi | tER | ER | |
| Endogenous dGRASPa | |||||||
| Salivary glands | 77.1 ± 3.0 | 33.9 ± 2.5 | 40.0 ± 1.3 | 26.1 ± 1.7b | 0.84 | 0.53 | 0.12 |
| S2 cells | 69.4 ± 6.8 | 46.7 ± 9.2 | 33.4 ± 3.9 | 19.9 ± 8.6 | |||
| dGRASP-GFP | |||||||
| Salivary glands | 74.7 ± 7.3 | 52.6 ± 8.9 | 30.7 ± 7.7 | 16.7 ± 6.8 | |||
| S2 cells | 78.4 ± 6.9 | 53.6 ± 11.9 | 30.1 ± 10.2 | 16.3 ± 7.8 | |||
a
Data obtained from S2 cells and salivary glands with the anti-GRASP65 antibody.
b
The increased percentage of gold labeling on the ER is due to its large surface section in salivary glands, making the background contribution higher than for the other smaller organelles.